A Mixed Flavonoid-Fish Oil Supplement Induces Immune-Enhancing and Anti-Inflammatory Transcriptomic Changes in Adult Obese and Overweight Women – 2016
Lynn Cialdella-Kam, Sujoy Ghosh et al
– Flavonoids and fish oils have anti-inflammatory and immune-modulating influences.
– Determine if a mixed flavonoid-fish oil supplement would reduce complications associated with obesity; that is, reduce inflammatory and oxidative stress markers and alter genomic profiles in overweight women.
– Overweight and obese women (n = 48; age = 40-70 yrs) were assigned to Q-Mix or placebo groupsfor 10 weeks; Q-Mix:
(a) 1000 mg quercetin, 400 mg isoquercetin, 120 mg epigallocatechin (EGCG) from green tea extract
(b) 400 mg n3-PUFAs omega-3 polyunsaturated fatty acid: 220 mg eicosapentaenoic acid (EPA); 180 mg docosahexaenoic acid (DHA)) from fish oil
(c) 1000 mg vitamin C, 40 mg niacinamide
(d) 800 µg folic acid)
(1) Plasma levels increased with Q-Mix supplementation by 388% for quercetin, 95% for EPA, 18% for DHA, and 20% for docosapentaenoic acid (DPA)
(2) Q-Mix did not alter plasma levels for CRP, F2-isoprostanes, and cytokines
(2) Gene set enrichment analysis revealed upregulation of pathways in Q-Mix vs. placebo related to interferon-induced antiviral mechanism
(3) Overrepresentation analysis further disclosed an inhibition of phagocytosis-related inflammatory pathways in Q-Mix vs. placebo.
– supplementation elicited a significant rise in plasma quercetin, EPA, DHA, and DPA
– stimulated an antiviral and inflammation whole-blood transcriptomic response in overweight women.
Carl J. Ade, S. K. Rosenkranz, C. A. Harms
– Determine if omega-3 via fish oil would mitigate airway inflammatory response induced by a single High Fat Meal – HFM
(1) 17 non-asthmatic men (22±2yrs) were supplemented with 3g/day fish oil or a placebo for 3wks
(2) measurements taken prior to and 2hr following a HFM.
(1) Following a HFM, triglycerides increased in both fish oil and placebo groups compared to pre-HFM (~59 and ~49 %, respectively
(2) The percent increase in FENO was greater in the placebo group compared to the fish oil group (25.7 ± 16.7 vs. −1.99 ± 10.5 %, respectively)
(3) A significant correlation was observed between blood triglycerides and FENO in the placebo group, but not the fish oil group
– A single HFM increases airway inflammation and omega-3 via fish oil protects against HFM associated changes in airway health